Biostimulation with oxygen and electron donors supports micropollutant biodegradation in an experimentally simulated nitrate-reducing aquifer.

The availability of suitable electron donors and acceptors limits micropollutant natural attenuation in oligotrophic groundwater. This study investigated how electron donors with different biodegradability (humics, dextran, acetate, and ammonium), and different oxygen concentrations affect the biodegradation of 15 micropollutants (initial concentration of each micropollutant = 50 µg/L) in simulated nitrate reducing aquifers. Tests mimicking nitrate reducing field conditions showed no micropollutant biodegradation, even with electron donor amendment. However, 2,4-dichlorophenoxyacetic acid and mecoprop were biodegraded under (micro)aerobic conditions with and without electron donor addition. The highest 2,4-dichlorophenoxyacetic acid and mecoprop biodegradation rates and removal efficiencies were obtained under fully aerobic conditions with amendment of an easily biodegradable electron donor. Under microaerobic conditions, however, amendment with easily biodegradable dissolved organic carbon (DOC) inhibited micropollutant biodegradation due to competition between micropollutants and DOC for the limited oxygen available. Microbial community composition was dictated by electron acceptor availability and electron donor amendment, not by micropollutant biodegradation. Low microbial community richness and diversity led to the absence of biodegradation of the other 13 micropollutants (such as bentazon, chloridazon, and carbamazepine). Finally, adaptation and potential growth of biofilms interactively determined the location of the micropollutant removal zone relative to the point of amendment. This study provides new insight on how to stimulate in situ micropollutant biodegradation to remediate oligotrophic groundwaters as well as possible limitations of this process.

Impact of aerobic granular sludge sizes and dissolved oxygen concentration on greenhouse gas N2O emission.

Aerobic granular sludge (AGS) at wastewater treatment plants (WWTPs) are known to produce nitrous oxide (N2O), a greenhouse gas which has a ∼300 times higher global warming potential than carbon dioxide. In this research, we studied N2O emissions from different sizes of AGS developed at a dissolved oxygen (DO) level of 2 mgO2/L while exposing them to disturbances at various DO concentrations ranging from 1 to 4 mgO2/L. Five different AGS size classes were studied: 212-600 µm, 600-1000 µm, 1000-1400 µm, 1400-2000 µm, and > 2000 µm. Metagenomic data showed N2O reductase genes (nosZ) were more abundant in the smaller AGS sizes which aligned with the observation of higher N2O reduction rates in small AGS under anaerobic conditions. However, when oxygen was present, the activity measurements of N2O emission showed an opposite trend compared to metagenomic data, smaller AGS (212 to 1000 µm) emitted significantly higher N2O (p < 0.05) than larger AGS (1000 µm to >2000 µm) at DO of 2, 3, and 4 mgO2/L. The N2O emission rate showed positive correlation with both oxygen levels and nitrification rate. This pattern indicates a connection between N2O emission and nitrification. In addition, the data suggested the penetration of oxygen into the anoxic zone of granules might have hindered nitrous oxide reduction, resulting in incomplete denitrification stopping at N2O and consequently contributing to an increase in N2O emissions. This work sets the stage to better understand the impacts of AGS size on N2O emissions in WWTPs under different disturbance of DO conditions, and thus ensure that wastewater treatment will comply with possible future regulations demanding lowering greenhouse gas emissions in an effort to combat climate change.

Repeated hypoxic episodes allow hematological and physiological habituation in rainbow trout.

Introduction: Under climate change, the increase in temperature in aquatic environments may induce oxygen depletion. In extreme cases, low oxygen may become a limiting factor for fish, thus generating stress. In addition, consecutive hypoxic episodes may complicate the recovery of individuals and hinder their ability to modulate physiological and biochemical responses to maintain homeostasis. Thus, the aim of this study was to determine the hematological and physiological responses of rainbow trout under a condition of repeated hypoxic and manipulation stresses at three different time points. Methods: Every hypoxic episode consisted of exposing the fish to low dissolved oxygen concentrations (2 mgO2/L for 1 h). Following the exposure, the fish were allowed to recover for 1 h, after which they were sampled to investigate hematological and physiological parameters. Results and discussion: The results showed a pattern of habituation reflected by values of hematocrit, hemoglobin, and mean corpuscular volume, indicating a certain ability of rainbow trout to resist this type of repeated hypoxic events, provided that the fish can have some recovery time between the exposures.

Spatiotemporal variation of dissolved oxygen concentration in the northern Gulf of Mexico during the period of 1992-2017.

Dissolved oxygen (DO) concentration is an important index of ocean systems. In this study, spatiotemporal variations in DO were analyzed in the northern Gulf of Mexico (NGM) for the period of 1992-2017. Temporal variation involves annual, monthly, and seasonal variations. The spatial scope ranged from the sea surface to 1000 m depth. The results show that: (1) DO decreased from 1992 to 2017 with a brief increase; (2) clear seasonal variation characteristics of DO were presented, and the seasonal average DO was relatively low in summer; (3) the monthly average trends of sea surface DO in different years were relatively consistent; and (4) with increasing water depth from seawater surface to 1000 m, the average DO of 1992 to 2017 tended to decrease and then increase. The results aim at shedding some light on the assessment of DO change in the NGM over the past quarter century.

Effect of dissolved oxygen concentration on performance and mechanism of simultaneous nitrification and denitrification in integrated fixed-film activated sludge sequencing batch reactors.

Integrated fixed-film activated sludge (IFAS) is a superior system for achieving simultaneous nitrification and denitrification (SND), however, the impact of dissolved oxygen (DO) has not been fully elucidated. Therefore, this study investigated the effect of DO concentration on performance and mechanism of SND in IFAS system. Results showed that IFAS outperformed control systems and achieved optimal SND performance at a DO concentration of 0.5 mg/L, with an SND efficiency of 88.51% and total nitrogen removal efficiency of 82.78%. Typical cycles analysis demonstrated limited-DO promoted SND performance. Further analysis implied biofilms exhibited high biomass and denitrification activity with decreasing DO. Microbial community analysis revealed low DO concentrations were responsible for abundant functional groups and genes associated with SND and promoted unconventional nitrogen removal pathways. Moreover, co-occurrence network analysis elucidated microbial interactions, responses to DO, and keystone genera. This study helps understanding the roles of DO for enhanced SND in IFAS.

Simulation and experimental verification of nitrite-oxidizing bacteria inhibition by alternating aerobic/anoxic strategy.

Anaerobic ammonium oxidation (ANAMMOX) is a promising technology for sewage treatment. Alternating aerobic/anoxic conditions have been widely adopted to achieve partial nitrification (PN), so as to provide substrates for ANAMMOX. In this study, the feasibility of PN with the strategy of intermittent aeration was investigated under mainstream conditions. At a low dissolved oxygen (DO) concentration, the nitrogen conversion characteristic under different intermittent aeration modes was evaluated by mathematical simulation and experimental method with (1) ordinary activated sludge, (2) mixed sludge with anaerobic ammonia-oxidizing bacteria (AnAOB), and (3) PN sludge, as seed sludge. The existence of functional microorganisms, such as AnAOB and denitrifying bacteria, which can utilize nitrites, was the prerequisite for NOB activity inhibition in the alternating aerobic/anoxic condition. Therefore, low nitrite may be an important factor in NOB activity inhibition under alternating aerobic/anoxic conditions. This study demonstrated a key controlling factor for NOB activity inhibition with alternating aerobic/anoxic condition.

Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor.

A bioreactor is a controlled vessel which provides biological conversions into bioactive components using cells or enzymes. In the aerobic processes, it is important to know oxygen requirements of the cells which may vary during fermentation as a result of microbial activity, aging, substrate depletion and product formation, etc. Here we describe the measurement of volumetric mass transfer coefficient (k L a) in a stirred tank reactor using dynamic method based on unsteady state which is also one of the significant parameter especially in scaling-up. The equipment in the measurement according to dynamic method has low cost compared to steady-state methodology. This method is reliable in the determination of k L a when the gas residence time and probe measuring the oxygen concentration of response time are in specific requirements.

Dissolved oxygen variation in the North China Plain river network region over 2011-2020 and the influencing factors.

The 2011-2020 data of dissolved oxygen (DO) and nutrients-total phosphorus (TP), solution reactive phosphorus (SRP), total nitrogen (TN), ammoniacal nitrogen (NH3-N), and chemical oxygen demand (CODCr)-in the North China Plain river network region were analyzed. Moreover, the DO variation trend and the influencing factors were investigated. The results showed that between 2011 and 2020, the DO concentration steadily increased from <3 mg L-1 to >5 mg L-1. Negative relationships were observed between the DO and CODCr (R = -0.34, p < 0.01), TN (R = -0.41, p < 0.01), NH3-N(R = -0.40, p < 0.01), TP (R = -0.28, p < 0.01), and SRP (R = -0.19, p < 0.01), indicating that a reduction in the nutrient input promoted the increase in the DO concentration in the past decade. The DO concentration in the rainy season was lower than that in the dry season, which suggests that nonpoint-source pollution caused by heavy storm runoff was the main factor affecting the water quality. The average DO concentration in the suburban rivers (4.88 mg L-1) was higher than that in urban rivers (3.41 mg L-1). Furthermore, comprehensive analysis indicated that the loss of riparian buffer, river solidification, pollutant input, and sluice dam operations are the main factors affecting DO concentration decrease and water quality deterioration. Finally, measures for water improvement and DO recovery in the river network region, namely pollutant input control, reduction in the impact of water conservancy projects, and river ecological restoration, were examined. Overall, this study shows that water quality has improved over the past decade. The study results provide a reference for the continuous improvement of water quality and the continuous recovery of DO in river network regions.

Practical Considerations of Dissolved Oxygen Levels for Platelet Function under Hypoxia.

Investigating human platelet function in low-oxygen environments is important in multiple settings, including hypobaric hypoxia (e.g., high altitude), sea level hypoxia-related disease, and thrombus stability. These studies often involve drawing blood from which platelets are isolated and analysed at atmospheric conditions or re-exposed to low oxygen levels in hypoxia chambers before testing. However, it remains unknown how the in vitro handling of the samples itself changes their dissolved oxygen concentration, which might affect platelet function and experimental results. Here, we prepared healthy donor platelet-rich plasma and washed platelet (WP) suspensions and exposed them to 2% oxygen. We found that the use of hypoxia pre-equilibrated tubes, higher platelet concentrations (>2 × 108/mL versus 2 × 107/mL), smaller volumes (600 µL versus 3 mL), and presence of plasma reduced the time for samples to reach 2% oxygen. Notably, oxygen levels decreased below 2% in most suspensions, but also in WP maintained at atmospheric 21% oxygen. Additionally, platelet spreading on fibrinogen was decreased when using hypoxic fibrinogen-coated culture plates regardless of the oxygen percentage (2% or 21%) in which platelet incubation took place. Thus, sample handling and experimental conditions should be carefully monitored in platelet-hypoxia studies as they might compromise results interpretation and comparison across studies.

An efficient strategy for predicting river dissolved oxygen concentration: application of deep recurrent neural network model.

Dissolved oxygen (DO) concentration in water is one of the key parameters for assessing river water quality. Artificial intelligence (AI) methods have previously proved to be accurate tools for DO concentration prediction. This study presents the implementation of a deep learning approach applied to a recurrent neural network (RNN) algorithm. The proposed deep recurrent neural network (DRNN) model is compared with support vector machine (SVM) and artificial neural network (ANN) models, formerly shown to be robust AI algorithms. The Fanno Creek in Oregon (USA) is selected as a case study and daily values of water temperature, specific conductance, streamflow discharge, pH, and DO concentration are used as input variables to predict DO concentration for three different lead times ("t + 1," "t + 3," and "t + 7"). Based on Pearson's correlation coefficient, several input variable combinations are formed and used for prediction. The model prediction performance is evaluated using various indices such as correlation coefficient, Nash-Sutcliffe efficiency, root mean square error, and mean absolute error. The results identify the DRNN model ([Formula: see text]) as the most accurate among the three models considered, highlighting the potential of deep learning approaches for water quality parameter prediction.

Effects of sub-atmospheric pressure and dissolved oxygen concentration on lesions generated in ex vivo tissues by high intensity focused ultrasound.

BACKGROUND: Acoustic cavitation plays an important role in the medical treatment using high-intensity focused ultrasound (HIFU), but unnecessarily strong cavitation also could deform the morphology and enlarge the size of lesions. It is known that the increase of ambient hydrostatic pressure (Pstat) can control the acoustic cavitation. But the question of how the decrease of Pstat and dissolved oxygen concentration (DOC) influence the strength of cavitation has not been thoroughly answered. In this study, we aimed to investigate the relationship among the Pstat, DOC and the strength of cavitation. METHODS: Ex vivo bovine liver tissues were immersed in degassed water with different DOC of 1.0 mg/L, 1.5 mg/L and 2.0 mg/L. Ultrasound (US) of 1 MHz and the spatial and temporal average intensity (Isata) of 6500 W/cm2 was used to expose two groups of in vitro bovine livers for 2 s; one group was under atmospheric pressure (Pstat = 1 bar) and the other was under sub-atmospheric pressure (Pstat = 0.1 bar). Acoustic cavitation was detected by a passive cavitation detector (PCD) during the exposure process. Echo signals at the focal zone of HIFU were monitored by B-mode ultrasound imaging before and after exposure. The difference between two pressure groups was tested using paired sample t-test. The difference among different DOC groups was evaluated by one-way analysis of variance (ANOVA). RESULTS: The results demonstrated a significant difference of broadband acoustic emissions from the cavitation bubbles, echo signals on B-mode image, morphology of lesions under various conditions of ambient pressure and DOC. The lesion volume in tissue was increased with the increase of ambient pressure and DOC. CONCLUSION: Cavitation could be suppressed through sub-atmospheric pressure and low DOC level in liver tissue, which could provide a method of controlling cavitation in HIFU treatment to avoid unpredictable lesions.

Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.

Fructo-oligosaccharides (FOS) are prebiotics with numerous health benefits. So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown. In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study. The greatest FOS production (123.2 ± 6.2 g/L), with a yield of 61.6% ± 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration. Furthermore, repeated-batch culture revealed that enzyme production and FOS production were not closely associated with cell growth. By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 ± 37.4 g/L and yield of 68.6% ± 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively. Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture. Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.

Influence of activated sludge dissolved oxygen concentration on a membrane bioreactor performance with intermittent aeration.

This study measured the effect of low activated sludge dissolved oxygen (DO) concentration on a membrane bioreactor (MBR) treating real urban wastewater with respect to organic matter and nitrogen removal efficiency and transmembrane pressure evolution. For this purpose, a full-scale experimental pre-denitrification MBR system was operated at a constant permeate flow rate of Q = 0.45 m3h-1 with intermittent aeration. The experimental installation worked at high hydraulic retention time, variable sludge retention time and with activated sludge temperatures of between 22.0 to 31.3 °C. Mean DO concentrations in the activated sludge were gradually decreased from 1.25 mgO2L-1 to less than 0.20 mgO2L-1. Variations in DO set points did not affect the main operational parameters of the MBR system and no clear relation was shown between DO concentration decrease and membrane fouling. At DO concentrations lower than 0.2 mgO2L-1, a deterioration in MBR effluent quality was observed, mainly with respect to chemical oxygen demand, biochemical oxygen demand at five days and NH4+, however, the opposite effect was observed for NO3-. These results indicate that employing low DO set points is a promising strategy for application in MBR systems.

Nest-site selection in a fish species with paternal care.

Fish that perform paternal care may increase their fitness by choosing nest sites that enhance survival and development of embryos. We studied nest-site choice with respect to dissolved oxygen concentration and water temperature in males of the three-spined stickleback (Gasterosteus aculeatus), a small fish species with exclusive male parental care that usually breeds in the littoral zone of freshwaters of the Northern hemisphere. Fathers oxygenate the embryos by fanning movements of their pectoral fins. We expected choice for conditions at potential nest sites that would benefit offspring development, i.e., higher temperature and higher dissolved oxygen concentration. In the laboratory, we offered males a choice between two potential nest sites that differed in dissolved oxygen concentration or water temperature. Males preferred to build a nest at sites with a higher dissolved oxygen level or higher temperature and thus chose sites that would promote embryo development.

Influence of Oxygen Supply on Growth and Laccases Production by Pleurotus sajor-caju PS-2001 in Submerged Process

Abstract (1) Background: Oxygen supply is an important parameter to be considered in submerged cultures. This study evaluated the influence of different conditions for dissolved oxygen (DO) concentration on laccases activities and growth of Pleurotus sajor-caju PS-2001 in submerged process in stirred-tank bioreactor. (2) Methods: Initially, three different conditions were tested: uncontrolled DO and minimum levels of 30% and 80% of saturation, with the pH controlled between 4.5 and 7.0. (3) Results: Best results were observed at 30% DO (26 U mL-1 of laccases at 96 h), whereas higher mycelial biomass was observed at 30% and 80% DO (above 4.5 g L-1). Four different conditions of DO (uncontrolled, 10%, 30% and 50% of saturation) were tested at pH 6.5, with higher laccases activity (80 U mL-1 at 66 h) and lower mycelial growth (1.36 g L-1 at 90 h) being achieved with DO of 30%. In this test, the highest values for volumetric productivity and specific yield factor were determined. Under the different pH conditions tested, the production of laccases is favoured at DO concentration of 30% of saturation, while superior DO levels favours fungal growth. (4) Conclusion: The results indicate that dissolved oxygen concentration is a critical factor for the culture of P. sajor-caju PS-2001 and has important effects not only on laccases production but also on fungal growth.

Economical lipid production from Trichosporon oleaginosus via dissolved oxygen adjustment and crude glycerol addition.

The effect of dissolved oxygen concentration on lipid accumulation in Trichosporon oleaginosus has been investigated. The experiment was performed in 15 L fermenters. The dissolved oxygen concentration varied by adjusting the agitation and aeration. High dissolved oxygen level at 50%-60% enhanced cell growth. Maintaining low dissolved oxygen concentration at 20%-30% during lipogenesis phase led to high final lipid content (51%) in Trichosporon oleaginosus. The consumptions of energy and cost of the process were evaluated. The energy consumption in the dissolved oxygen level optimized process was 41% less than that with dissolved oxygen level at 50%-60%. In addition, the cost was also reduced around one time in the dissolved oxygen level optimized process compared to the one with dissolved oxygen level at 50%-60%. The study provided a feasible way of enhancing lipid accumulation in Trichosporon oleaginosus and reducing the consumption of energy and cost of lipid production from Trichosporon oleaginosus.

Evaluation of the impact of dissolved oxygen concentration on biofilm microbial community in sequencing batch biofilm reactor.

The effect of dissolved oxygen concentration (DO) during simultaneous nitrification and denitrification (SND) was investigated in a sequencing batch biofilm reactor (SBBR). In addition, the removal rates of nitrogen and bacterial communities were investigated under different concentrations of DO (1.5, 3.5, and 4.5 mg/L). When the SND rate was 95.22%, the chemical oxygen demand and nitrogen removal was 92.22% and 84.15%, respectively, at 2.5 mg/L DO. The denitrification was inhibited by the increase of oxygen concentration. Microelectrode measurements showed that the thickness of oxygen penetration increased from 1.0 mm to 2.7 mm when the DO concentration increased from 1.5 mg/L to 5.5 mg/L. The current location of the aerobic and anaerobic layers in the biofilm was determined for analysis of the microbial community. High-throughput sequencing analysis revealed the communities of the biofilm approached similar structure and composition. Uliginosibacterium species, biofilm-forming bacteria Zoogloea species and Acinetobacter species were dominant. In the aerobic layer, phyla Betaproteobacteria and Saprospirae were predominant, the major phyla were shifted from Proteobacteria followed by Firmicutes and Bacteroidetes, which comprised 82% of the total sequences during the SND period. Anaerolineae was dominated in the anaerobic layer. The high abundance of Nitrospira in the aerobic biofilm provides evidence of the SND system performing better at ammonia oxidization. In addition, real-time PCR indicated that the amount of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) matched the Nitrospirales and Nitrosomonadales abundance well. Collectively, this study demonstrated the dynamics of key bacterial communities in the SND system were highly influenced by the DO concentration.

Hydrogen productivity of hydrogen-rich sticks increased by ammonium salt / 军事医学

Objective To measure the catalytic effect of ammonium salt solution on hydrogen production , and analyze the relationships between hydrogen concentration and oxygen concentration , oxidation-reduction potential , and pH in solution . Methods Magnesium and water reacted at different concentrations of NH4Cl, (NH4)2SO4, NH4HCO3,(NH4)2CO3, NaHCO3 and Na2SO3 in 40℃water bath for 0, 2, 4, 6, 8 and 10 hours, and above-mentioned four indicators of ammonium salt solution were measured after these reactions .Results The hydrogen concentration in the solution increased with the reaction time and the concentration of the ammonium salt solution .Oxygen concentration and oxidation-reduction potential decreased .The hydrogen concentration in the solution was significantly negatively correlated with the oxygen concentration (r=-0.984).pH increased with the hydrogen concentration in the ammonium salt solution .Conclusion The ammonium salt solution has a good catalytic effect on magnesium and water reaction .NH4Cl has the strongest catalytic effect under the same reaction conditions,followed by (NH4)2SO4, NH4HCO3 and (NH4)2CO3.Thus, this study can provide detailed data on hydrogen production in different solutions .

Influence of oxygen availability on the metabolism and morphology of Yarrowia lipolytica: insights into the impact of glucose levels on dimorphism.

Dynamic behavior of Yarrowia lipolytica W29 strain under conditions of fluctuating, low, and limited oxygen supply was characterized in batch and glucose-limited chemostat cultures. In batch cultures, transient oscillations between oxygen-rich and -deprived environments induced a slight citric acid accumulation (lower than 29 mg L-1). By contrast, no citric acid was detected in continuous fermentations for all stress conditions: full anoxia (zero pO2 value, 100% N2), limited (zero pO2 value, 75% of cell needs), and low (pO2 close to 2%) dissolved oxygen (DO) levels. The macroscopic behavior (kinetic parameters, yields, viability) of Y. lipolytica was not significantly affected by the exposure to DO fluctuations under both modes of culture. Nevertheless, conditions of oxygen limitation resulted in the destabilization of the glucose-limited growth during the continuous cultivations. Morphological responses of Y. lipolytica to DO oscillations were different between batch and chemostat runs. Indeed, a yeast-to-mycelium transition was induced and progressively intensified during the batch fermentations (filamentous subpopulation reaching 74% (v/v)). While, in chemostat bioreactors, the culture consisted mainly of yeast-like cells (mean diameter not exceeding 5.7 µm) with a normal size distribution. During the continuous cultures, growth at low DO concentration did not induce any changes in Y. lipolytica morphology. Dimorphism (up to 80.5% (v/v) of filaments) was only detected under conditions of oxygen limitation in the presence of a residual glucose excess (more than 0.75 g L-1). These data suggest an impact of glucose levels on the signaling pathways regulating dimorphic responses in Y. lipolytica.

The effect of dissolved oxygen concentration (DO) on oxygen diffusion and bacterial community structure in moving bed sequencing batch reactor (MBSBR).

The effect of dissolved oxygen concentration (DO) on simultaneous nitrification and denitrification was studied in a moving bed sequencing batch reactor (MBSBR) by microelectrode measurements and by real-time PCR. In this system, the biofilm grew on polyurethane foam carriers used to treat municipal sewage at five DO concentrations (1.5, 2.5, 3.5, 4.5 and 5.5 mg/L). The results indicated that the MBSBR exhibited good removal of chemical oxygen demand (92.43%) and nitrogen (83.73%) when DO concentration was 2.5 mg/L. Increasing the oxygen concentration in the reactor was inhibitory to denitrification. Microelectrode measurements showed that the thickness of oxygen penetration increased from 1.2 to 2.6 mm when the DO concentration (from 1.5 mg/L to 5.5 mg/L) in the system increased. Oxygen diffusion was not significantly limited by the boundary layer surrounding the carrier and had the largest slope when DO concentration was 2.5 mg/L. The real-time PCR analysis indicated that the amount of the ammonia-oxidizing bacteria and nitrite-oxidizing bacteria increased slowly as DO concentration increased. The proportions of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria, as a percentage of the total bacteria, were low with average values of 0.063% and 0.67%, respectively. When the DO concentration was 2.5 mg/L, oxygen diffusion was optimal and ensured the optimal bacterial community structure and activity; under these conditions, the MBSBR was efficient for total inorganic nitrogen removal. Changing the DO concentration could alter the aerobic zone and the bacterial community structure in the biofilm, directly influencing the simultaneous nitrification and denitrification activity in MBSBRs.